Fluorescence Spectroscopy Question & Answers December 16, 2022 By WatElectronics This article lists 75 Fluorescence Spectroscopy MCQs for engineering students. All the Fluorescence Spectroscopy Questions & Answers below include a hint and a link to the relevant topic wherever possible. This is helpful for users who are preparing for their exams, interviews, or professionals who would like to brush up on the fundamentals of Fluorescence Spectroscopy. Electro-magnetic spectroscopy that can analyze fluorescence present in the sample is referred to as Fluorescence Spectroscopy. Spectrofluorometry or fluorometry are the other names for this spectroscopy. The light beam is involved to excite electrons present in molecules in spectroscopy. Usually, it prefers ultra-violet light in Fluorescence Spectroscopy to excite electrons in the molecules and cause the light to emit from them. Molecules contain distinct states referred to as Energy levels. Primarily, Fluorescence Spectroscopy deals with the vibrational & electronic states of molecules. Absorbing of photons excites the species in fluorescence. Hence, the vibrational energy of a present molecule is lost due to colliding with the other molecule. This type of process can be visualized through the Jablonski diagram. Specificity and sensitivity are high in Fluorescence-based spectroscopy. Fluorescence spectroscopy is preferable to analyze compounds that are organic in the fields of chemical, medical & biochemical for the purpose of research. To differentiate malignant tumors of skin from benign ones this spectroscopy is used. In addition to it, water contamination due to bacteria can also be detected using Spectroscopy of fluorescence. 1). Fluorescence spectroscopy is _______ spectroscopy? Electric Physical Mechanical Electromagnetic None Hint 2). Which light is utilized in fluorescent spectroscopy? Infrared Ultraviolet Laser Radar None Hint 3). Absorption spectroscopy is complementary of ________ spectroscopy? Fluorescence Chemiluminescence Electroluminescence All Mentioned Above None Hint 4). Device that measures fluorescence are ______? Phyto-meters Spirometers Fluorometers Potentiometers None Hint 5). For what states fluorescence spectroscopy is concerned? Electronic States Chemical States Vibrational States a & c None Hint 6). How does the species get excited in fluorescence? By Absorbing Proton By Absorbing Nucleon By Absorbing Temperature By Absorbing Photon None Hint 7). Absorbed photon if it is re-emitted the process is referred to as _______? Scattering Fluorescence Chemical Fluorescence Non-resonance Fluorescence Resonance Fluorescence None Hint 8). What will be fixed while measuring fluorescence? Emission Wavelength Excitation Wavelength Photon Wavelength Electron Wavelength None Hint 9). What happens to the wavelength of detection during excitation of measuring fluorescence? Fixed Dynamic Varying Continuously May or May not Change None Hint 10) . What are the instruments that distinguish the light of incidence & fluorescence? Filter Fluorometers Spectro Fluorometers Noise Fluorometers a & b None Hint 11). Fluorescence is a kind of _________________? Photoluminescence Chemiluminescence Electroluminescence All Mentioned Above None Hint 12). Fluorescence spectrum of excitation has _________________ fixed? Emission Wavelength Excitation Wavelength Photon Wavelength Electron Wavelength None Hint 13). Emission spectrum of Fluorescence has ___________ fixed? Emission Wavelength Excitation Wavelength Photon Wavelength Electron Wavelength None Hint 14). How the sample is changed noticed by? Emission Excitation Environment a & b None Hint 15). Spectral intensity may change due to _______________? Temperature Molecules Concentration Interaction with Other Molecules All Mentioned Above None Hint 16). Organic molecules of fluorescent have ______________? Aromatic Rings Pi-conjugate Electrons Protons a & b None Hint 17). What are the spectrums measured based on fluorescence spectrometry? Emission Excitation Eradication a & b None Hint 18). Analytes concentration is _______________ to emissions intensity? Directly Proportional Inversely Proportional Constant Doesn’t Change None Hint 19). Wavelength of a sample excited effects ___________? Intensity Solution Frequency Path Length None Hint 20). What is dependent on cuvettes' path length? Intensity Solution Frequency Samples Self-absorption None Hint Fluorescence Spectroscopy MCQs for Interviews 21). How many analytes in the solution are analysed using Fluorescence Spectrometry? One More Than One Zero None None Hint 22). Analytes in Fluorescence Spectrometry must emit ____________? Visible Light Near IR radiation Ultra Violet Radiation a & b None Hint 23). What are the lights that must be absorbed for using Fluorescence Spectrometry? Ultra Violet Visible Infrared Ultra-violet & Visible None Hint 24). What are the solvents used in the analysis of fluorescence? Water Ethanol Hexane All Mentioned Above None Hint 25). Samples self-absorption can affect _____________? Intensity Solution Frequency Path Length None Hint 26). If any molecule or atom absorbing the energy is referred to as ____________? Emission Excitation Enlargement Enhancement None Hint 27). After excitation of the molecule, __________________ is emitted in Fluorescence? Electron Proton Neutron Photon None Hint 28). Emission time in Fluorescence is ________________ order? Milli-seconds Micro-seconds Nano-seconds Pico-seconds None Hint 29). Change in ____________ affects the position & shape of the fluorescence spectrum of emission? Intensity Solvent Frequency Path Length None Hint 30). Molecules containing atoms of halogen reduce _____________? Light Emission Light Absorption Light Application Light Incidence None Hint 31). What atoms or ions cause fluorescent emissions quenching? Iodine Bromine Chlorine All Mentioned Above None Hint 32). Halogens are capable of eliminating excess energy in ____________ molecule? Photoluminescence Chemiluminescence Electroluminescence Fluorescence None Hint 33). FCS stands for _______________? Fluorescence Correlation Spectroscopy Fluorescence Communication System Fluorescence Compatible Statistics Fluorescence Computation Statistics None Hint 34). Light emitted in Fluorescence spectroscopy have ___________ energy? Lower Higher Severe Moderate None Hint 35). ___________ is utilized for temporal & spatial analysis of the molecule-level interactions? Fluorescence Correlation Spectroscopy Chemiluminescence Spectroscopy Electroluminescence Spectroscopy All Mentioned Above None Hint 36). What is measured using FCS? Average Number of Molecules Molecules Diffusion Time Molecules Detection Time a & b None Hint 37). Molecule's speed of diffusion is proportional to _____________? Molecular Mass Fluorescent Molecules Shape Molecule Consistency a & b None Hint 38). If the molecule is big then the time taken to diffuse is ___________ in Fluorescence spectroscopy? Faster Slower Doesn’t Melt Moderate None Hint 39). What is used in the extraction of several coefficients of diffusion in the particles of fluorescence? Auto Correlation Function Cross-Correlation Function Moderate Correlation Function Straight correlation Function None Hint 40). What calculates the particle's average time of diffusion inside FCS? Auto Correlation Function Cross-Correlation Function Moderate Correlation Function Straight correlation Function None Hint Fluorescence Spectroscopy MCQs for Students 41). What are the various methods present in molecules of fluorescent? Internal Conversion Intersystem Crossing External Conversion All Mentioned Above None Hint 42). Energy gap is ___________ in the internal conversions? Small Large More Infinite None Hint 43). Collison quenching leads to a loss in energy in ______________? Internal Conversion Intersystem Crossing External Conversion All Mentioned Above None Hint 44). Electronic transitions from a high state of electronic to of low energy happens in ______________? Internal Conversion Intersystem Crossing External Conversion All Mentioned Above None Hint 45). Energy transfer takes place to vibration mode in ____________? Internal Conversion Intersystem Crossing External Conversion All Mentioned Above None Hint 46). Fluorescence intensity decrease with ______________? Increase in Temperature Decrease in Temperature Independent of Temperature Always Constant None Hint 47). What happens if the levels of vibration get overlap with triplet & singlet states of excitation? Internal Conversion Intersystem Crossing External Conversion All Mentioned Above None Hint 48). Emitted photons if gets back to a state of the ground referred to as ___________? Photoluminescence Chemiluminescence Electroluminescence Fluorescence None Hint 49). What happens to relaxation if the absorption occurred is lesser than 10*-15 seconds? Faster Slower Doesn’t get Affected Moderate None Hint 50). How intensity of fluorescence relates to the intensity of light excitation? Directly Proportional Inversely Proportional Constant Doesn’t Change None Hint 51). What does a fluorometer consist of? Excitation Monochromator Emission Monochromator Environment Monochromator a & b None Hint 52). Fluorescence relates directly to _______________? Luminous Flux Measurement Efficiency Dependency on Instruments Design All Mentioned Above None Hint 53). Emission spectra do not depend on __________? Emission Wavelength Excitation Wavelength Photon Wavelength Electron Wavelength None Hint 54). What does the information provide by a scan of emission? Fluorophore Structure of Molecule Local Environment Temperature a & b None Hint 55). What can be a tool in virus detection? Fluorescence Chemiluminescence Electroluminescence All Mentioned Above None Hint 56). Energy differences amid emission & excitation wavelengths is referred to as ___________? Jacobian Shift Faraday Shift Gauss Shift Stokes Shift None Hint 57). What is impacted due to fluorophores Stokes Shift? Temperature Intensity Humidity Spectral Bandwidth None Hint 58). What limits the acceptable range of spectral bandwidth? Jacobian Shift Faraday Shift Gauss Shift Stokes Shift None Hint 59). What distorts the spectrum of fluorescence? Artifacts Arti crafts Light Incident Electron Excited None Hint 60). What are the scatters occurring in fluorescence? Rayleigh Scatter Second Order Scatter Raman Scatter All Mentioned Above None Hint Fluorescence Spectroscopy MCQs for Exams 61). What provides more amount of information in comparison to DNA & RNA? Fluorescence Chemiluminescence Electroluminescence All Mentioned Above None Hint 62). What scattering is at peak for excited wavelength? Rayleigh Scatter Second Order Scatter Raman Scatter First Order Scatter None Hint 63). What scattering is of higher orders? Rayleigh Scatter Second Order Scatter Raman Scatter First Order Scatter None Hint 64). Which scatter occurs because of solvents? Rayleigh Scatter Second Order Scatter Raman Scatter First Order Scatter None Hint 65). _______________ is applied in the successful understanding of Human Rhino Virus-receptor interactions. Fluorescence Chemiluminescence Electroluminescence All Mentioned Above None Hint 66). What can be used for Pseudomonad taxonomic purposes? Fluorescence Chemiluminescence Electroluminescence All Mentioned Above None Hint 67). What are the benefits of FCS? Low Concentration of Samples Less Amount of Samples More Amount of Samples a & b None Hint 68). What makes fluorescence spectroscopy preferable in the medical field? High Sensitivity High Specificity Rate Low Specificity Rate a & b None Hint 69). ____________ have the rapid and fast ability of diagnosis? Fluorescence Chemiluminescence Electroluminescence All Mentioned Above None Hint 70). Amount of Hg (Mercury) in the sample of environment water can be determined using ___________? Phosphorescence Chemiluminescence Electroluminescence Fluorescence None Hint 71). Untransparent samples are unsuitable for ____________ spectroscopy? Fluorescence Chemiluminescence Electroluminescence All Mentioned Above None Hint 72). Analytes with photo-chemical reaction does not suitable for ___________ spectroscopy? Fluorescence Chemiluminescence Electroluminescence All Mentioned Above None Hint 73). Compounds in the solution are measured using ____________. Fluorescence Chemiluminescence Electroluminescence All Mentioned Above None Hint 74). How Fluorescence spectrometry is advantageous? Simple Fast Inexpensive All Mentioned Above None Hint 75). Polymers with aromatics conjugated have _____________ properties? Chemical Physical Fluorescence Electric None Hint Please fill in the comment box below. Time's up
