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Fluorescence Spectroscopy Question & Answers

December 16, 2022 By WatElectronics

This article lists 75 Fluorescence Spectroscopy MCQs for engineering students. All the Fluorescence Spectroscopy Questions & Answers below include a hint and a link to the relevant topic wherever possible. This is helpful for users who are preparing for their exams, interviews, or professionals who would like to brush up on the fundamentals of Fluorescence Spectroscopy.

Electro-magnetic spectroscopy that can analyze fluorescence present in the sample is referred to as Fluorescence Spectroscopy. Spectrofluorometry or fluorometry are the other names for this spectroscopy. The light beam is involved to excite electrons present in molecules in spectroscopy.

Usually, it prefers ultra-violet light in Fluorescence Spectroscopy to excite electrons in the molecules and cause the light to emit from them.  Molecules contain distinct states referred to as Energy levels. Primarily, Fluorescence Spectroscopy deals with the vibrational & electronic states of molecules.

Absorbing of photons excites the species in fluorescence. Hence, the vibrational energy of a present molecule is lost due to colliding with the other molecule. This type of process can be visualized through the Jablonski diagram. Specificity and sensitivity are high in Fluorescence-based spectroscopy.

Fluorescence spectroscopy is preferable to analyze compounds that are organic in the fields of chemical, medical & biochemical for the purpose of research. To differentiate malignant tumors of skin from benign ones this spectroscopy is used. In addition to it, water contamination due to bacteria can also be detected using Spectroscopy of fluorescence.

1). Fluorescence spectroscopy is _______ spectroscopy?

Hint
2). Which light is utilized in fluorescent spectroscopy?

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3). Absorption spectroscopy is complementary of ________ spectroscopy?

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4). Device that measures fluorescence are ______?

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5). For what states fluorescence spectroscopy is concerned?

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6). How does the species get excited in fluorescence?

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7). Absorbed photon if it is re-emitted the process is referred to as _______?

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8). What will be fixed while measuring fluorescence?

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9). What happens to the wavelength of detection during excitation of measuring fluorescence?

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10) . What are the instruments that distinguish the light of incidence & fluorescence?

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11). Fluorescence is a kind of _________________?

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12). Fluorescence spectrum of excitation has _________________ fixed?

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13). Emission spectrum of Fluorescence has ___________ fixed?

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14). How the sample is changed noticed by?

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15). Spectral intensity may change due to _______________?

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16). Organic molecules of fluorescent have ______________?

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17). What are the spectrums measured based on fluorescence spectrometry?

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18). Analytes concentration is _______________ to emissions intensity?

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19). Wavelength of a sample excited effects ___________?

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20). What is dependent on cuvettes' path length?

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Fluorescence Spectroscopy MCQs for Interviews

21). How many analytes in the solution are analysed using Fluorescence Spectrometry?

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22). Analytes in Fluorescence Spectrometry must emit ____________?

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23). What are the lights that must be absorbed for using Fluorescence Spectrometry?

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24). What are the solvents used in the analysis of fluorescence?

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25). Samples self-absorption can affect _____________?

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26). If any molecule or atom absorbing the energy is referred to as ____________?

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27). After excitation of the molecule, __________________ is emitted in Fluorescence?

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28). Emission time in Fluorescence is ________________ order?

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29). Change in ____________ affects the position & shape of the fluorescence spectrum of emission?

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30). Molecules containing atoms of halogen reduce _____________?

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31). What atoms or ions cause fluorescent emissions quenching?

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32). Halogens are capable of eliminating excess energy in ____________ molecule?

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33). FCS stands for _______________?

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34). Light emitted in Fluorescence spectroscopy have ___________ energy?

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35). ___________ is utilized for temporal & spatial analysis of the molecule-level interactions?

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36). What is measured using FCS?

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37). Molecule's speed of diffusion is proportional to _____________?

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38). If the molecule is big then the time taken to diffuse is ___________ in Fluorescence spectroscopy?

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39). What is used in the extraction of several coefficients of diffusion in the particles of fluorescence?

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40). What calculates the particle's average time of diffusion inside FCS?

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Fluorescence Spectroscopy MCQs for Students

41). What are the various methods present in molecules of fluorescent?

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42). Energy gap is ___________ in the internal conversions?

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43). Collison quenching leads to a loss in energy in ______________?

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44). Electronic transitions from a high state of electronic to of low energy happens in ______________?

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45). Energy transfer takes place to vibration mode in ____________?

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46). Fluorescence intensity decrease with ______________?

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47). What happens if the levels of vibration get overlap with triplet & singlet states of excitation?

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48). Emitted photons if gets back to a state of the ground referred to as ___________?

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49). What happens to relaxation if the absorption occurred is lesser than 10*-15 seconds?

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50). How intensity of fluorescence relates to the intensity of light excitation?

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51). What does a fluorometer consist of?

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52). Fluorescence relates directly to _______________?

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53). Emission spectra do not depend on __________?

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54). What does the information provide by a scan of emission?

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55). What can be a tool in virus detection?

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56). Energy differences amid emission & excitation wavelengths is referred to as ___________?

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57). What is impacted due to fluorophores Stokes Shift?

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58). What limits the acceptable range of spectral bandwidth?

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59). What distorts the spectrum of fluorescence?

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60). What are the scatters occurring in fluorescence?

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Fluorescence Spectroscopy MCQs for Exams

61). What provides more amount of information in comparison to DNA & RNA?

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62). What scattering is at peak for excited wavelength?

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63). What scattering is of higher orders?

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64). Which scatter occurs because of solvents?

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65). _______________ is applied in the successful understanding of Human Rhino Virus-receptor interactions.

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66). What can be used for Pseudomonad taxonomic purposes?

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67). What are the benefits of FCS?

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68). What makes fluorescence spectroscopy preferable in the medical field?

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69). ____________ have the rapid and fast ability of diagnosis?

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70). Amount of Hg (Mercury) in the sample of environment water can be determined using ___________?

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71). Untransparent samples are unsuitable for ____________ spectroscopy?

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72). Analytes with photo-chemical reaction does not suitable for ___________ spectroscopy?

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73). Compounds in the solution are measured using ____________.

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74). How Fluorescence spectrometry is advantageous?

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75). Polymers with aromatics conjugated have _____________ properties?

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